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SEROEPIDEMIOLOGICAL AND IMMUNO-VIROLOGICAL CHARACTERISATION OF PARVO B19 IgM AMONG FEBRILE HIV-INFECTED INDIVIDUALS IN CALABAR, NIGERIA

Etoroabasi Okpon Okpon¹, Blessing Jachinma Okonko², Edith Nnenna Oketah¹, Ann Onyinyechi Chukwu¹, Tochi Ifeoma Cookey¹ & Iheanyi Omezuruike Okonko¹

¹Virus & Genomics Research Unit, Department of Microbiology, University of Port Harcourt,           Choba, Rivers State, Nigeria. iheanyi.okonko@uniport.edu.ng; + (234) 7069697309:

²Medical Microbiology & Epidemiology Research Unit, Department of Microbiology, Madonna University Nigeria, Elele, Rivers State, Nigeria

Abstract: Human Parvovirus B19 (B19V) is an icosahedral single-stranded DNA virus which causes acute and persistent infections in immunocompromised individuals. Despite its potential impact, limited data is available on the seroepidemiological and immunovirological characterisation of Parvovirus B19 in febrile HIV individuals in Calabar, Nigeria. Therefore, this study is aimed at investigating the seroepidemiological and clinical manifestations of Parvovirus B19 IgM among febrile HIV-infected individuals in Calabar, Nigeria. The socio-demographic characteristics, as well as blood samples of 186 consenting individuals living with HIV presenting with febrile illness, were obtained and labelled accordingly, ruling out error. Following the manufacturer’s instructions on ELISA, Plasma was extracted and tested for Parvovirus B19 IgM antibodies. The results showed an overall prevalence of Parvovirus B19, 53.2% for IgM, indicating an ongoing infection. Higher IgM seropositivity was observed among males (61.9%), age group >51 (54.2%), married (53.4%), with primary education (63.6%), and public servants (100.0%). Based on clinical characteristics, a higher IgM prevalence occurred among individuals with viral load of ≥1000 copies/mL (92.9%) and CD4 cells of ≤ 200 cells/µL (68.4%), suggesting a strong association between recent B19V infection and virological failure. In conclusion, the study showed a high prevalence of Parvovirus B19 IgM among the studied population. The outcome of this study contributed to the understanding of the burden and clinical impact of Parvovirus B19 in HIV-positive patients. It informed the development of effective prevention and treatment strategies. These results enhanced knowledge of the variables that put these populations at risk for B19V infection.

[Okpon, E. O., Okonko, I. O., Oketah, E. N., Chukwu, A. O., Cookey, T. I. & Okonko, I. O.. Seroepidemiological and Immuno-Virological Characterisation of Parvo B19 among Febrile HIV-Infected Individuals in Calabar, Nigeria. Life Sci J 2026;23(6):1-9]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 01. doi:10.7537/marslsj230626.01

Keywords: Parvovirus B19; IgM; HIV; Seroepidemiological; Viral load; CD4 count; Calabar; Febrile illness

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Analysis of invitro regeneration potential of local Pakistani wheat genotypes using mature and immature zygotic embryos as explants

Farooq Saeed, Siddra Ijaz, Iqrar Ahmad Rana and Tariq Manzoor Khan

Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad Pakistan

Corresponding author: Siddra Ijaz, siddraijazkhan@yahoo.com

Abstract: We investigated the invitro regeneration potential of thirty two local wheat genotypes for genetic transformation purposes by using reported callus induction as well as regeneration media. Mature and immature zygotic embryos of these genotypes were used as explants. To study callus induction from mature embryos two callus induction media (CIM) were used. CIM1 contained MS salts with 2 mg/l 2, 4-D and 30 g/l sucrose while CIM2 contained 200 mg/l casein hydrolysate and 100 mg/l myoinositol in addition to CIM1. Thirty days old calli induced on CIM1 from mature embryo was shifted to Regeneration Medium-1 (RM1) which contained 0.1 mg/l 2,4-D along with MS salts and 30 g/l sucrose. Calli induced on CIM2 from mature embryos were shifted to Regeneration Medium-2 (RM2) having 0.5 mg/l BAP, 0.02 mg/l NAA, 200 mg/l Casein hydrolysate and 100 mg/l Myoinositol for regeneration. Calli were incubated at 26±1 ⁰C for 8-16 hrs dark and light condition. For callus induction from immature zygotic embryos only CIM1 was used. Thirty days old calli induced on CIM1 were shifted to RM1 and incubated at 26±1 °C for 8-16 hrs dark and light condition for regeneration. Our results showed that invitro regeneration is generally poor in most of Pakistani wheat genotypes tested. Luckily some genotypes showed better callus induction and regeneration ability. Among these genotypes AARI-2011 produced maximum, embryogenic calli at CIM1 from both mature and immature zygotic embryos and produced maximum number of shoots per explants on RM1. Next to AARI-2011 were 9372 and Lasani 2008. Regeneration from immature zygotic embryos was comparatively better than mature embryos.

[Farooq Saeed, Siddra Ijaz, Iqrar Ahmad Rana and Tariq Manzoor Khan. Analysis of invitro regeneration potential of local Pakistani wheat genotypes using mature and immature zygotic embryos as explants. Life Sci J 2026;23(6):10-31]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 02. doi:10.7537/marslsj230626.02

Keywords: Wheat; callus induction; immature zygotic embryo; Wheat Tissue culture; invitro regeneration

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Some factors affecting on nutritive value of whole plant corn silage

H.M.A. Gaafar

Animal Production Research Institute, Agricultural Research Center, Dokki, Egypt.

Email: gaafar356@hotmail.com

Abstract: In Egypt, the total planted area of corn crop was about 1 million feddans (420,000 hectare), but only 140 thousand feddans cultivated with yellow corn hybrids, the area of corn crop used as a silage was about 250 thousand feddans. Whole plant corn silage is a popular forage source for ruminants due to its high yielding properties, energy content, relatively high palatability and incorporating easily into TMR. Recently, agronomists, nutritionists, and dairy producers have placed increased emphasis on factors affecting the nutritive value of whole plant corn silage.

[H.M.A. Gaafar. Some factors affecting on nutritive value of whole plant corn silage. Life Sci J 2026;23(6):32-42]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 03. doi:10.7537/marslsj230626.03

Keywords: planted area; corn crop; corn hybrids; corn silage

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