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Life Science Journal 
Acta Zhengzhou University Overseas Edition
 (Life Sci J)
ISSN 1097-8135 (print); ISSN 2372-613X (online), doi:10.7537/j.issn.1097-8135, Monthly
 
Volume 12 - Number 7 (Cumulated No. 54), July 25, 2015. life1207
 Cover (oniline), Cover (print), Introduction, Contents, Call for Papers, Author Index, lsj1207
 

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CONTENTS  

No.

Titles / Authors /Abstracts

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1

Comparative study between conventional chemo-embolization and chemo-embolization using drug eluting beads as alternative methods for treatment of hepatocellular carcinoma

 

Randa S. Elshahat 1; Howida A. Ahmed1; Ola I. Saleh1; Eman E Amed Ibrahim 2 and Maisa A. Abdelwahab 3

 

1Radio-Diagnosis Department, Faculty of Medicine, Alazhar University

2Tropical department, Faculty of Medicine, Alazhar University

3Vascular surgery department, Faculty of Medicine, Alazhar University

oismail08@yahoo.com

 

Abstract: Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. HCC is associated with a poor prognosis and remains the third most common death-associated cancer due to the paucity of available treatment options. Combination of regional treatment has become arecent area of research and treatment advances.chemoembolization as an alternative treatment of HCC through achieving high, localized concentration of chemotherapeutic agents within the tumor as well induce localized ischemia by embolic material. These mixture of chemotherapeutic and embolic materials helped in reducing the tumor size and vascularity and so causing intr-tumeral necrosis. The aim of the work is to compare the effect of conventional chemo-embolization and chemo-embolization using drug eluting Beads as alternative methods for treatment of hepatocellular carcinoma. Patients & methods: Our study included 60 patients with HCC and classified into Group I: Included 26 patients underwent conventional chemoembolization and Group II: Included 34 patients underwent chemoembolization using drug eluting Beads. Results: In this study the response to treatment in Group I was seen in 17 patients (65%) while there was 9 patients (35%) non responding. In Group II there was response to treatment in 33 patients (97%) while there was one patient (3%) non responding to treatment there was statistical significant difference P=0.001. Conclusion: Chemo-embolization using drug eluting Beads is a promising method for treatment of HCC.

[Randa S. Elshahat; Howida A. Ahmed; Ola I. Saleh; Eman E Amed Ibrahim and Maisa A. Abdelwahab Comparative study between conventional chemo-embolization and chemo-embolization using drug eluting beads as alternative methods for treatment of hepatocellular carcinoma. Life Sci J 2015;12(7):1-8]. (ISSN:1097-8135). http://www.lifesciencesite.com. 1. doi:10.7537/marslsj120715.01

 

Keywords: DEC, Hepatocellular carcinoma &chemo-embolization

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Zoonotic Hazards of Campylobacteriosis in some areas in Egypt

 

Barakat A. M. A. 1; Mona M. Sobhy 2; El Fadaly H. A. A.1; Nagwa S. Rabie 3;, Nashwa O. Khalifa4;

Eman S. Ramadan2; Kotb, M. H. R.2, Zeinab M. S. Amin Girh3; Dalia M. Sedik3;and Mona S. Zaki5

 

1Zoonotic Diseases Department, National Research Centre, Dokki. 12622, Giza, Egypt.

2Department of Reproductive Diseases, ARRI, ARC, 12556, Giza, Egypt..

3Department of Poultry Diseases, National Research Centre, Dokki, 12622, Giza, Egypt.

4Zoonosis Department, Faculty of Veterinary Medicine, Benha University, Toukh, 13736, Egypt.

5 Hydrobiology Department, National Research Centre, Dokki, 12622, Giza, Egypt.

ashrafbarakat2@hotmail.com

 

Abstract: A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of patient with diarrhea from Menia, Fayoum, Cairo and Qaluobya in Egypt. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hippuricase gene. The overall prevalence of Campylobacter jejuni in intestine and liver of chicken were 40.4 % and 37.5 % respectively, 30% tape water, 4.44% raw milk, Karish cheese and yoghurt 6.66% and 13.33% respectively and 70 (35%) children stool. The positive results of C.jejuni were detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene of C. jejuni isolated from the clinically diseased chicken and the environmental samples have shown identical fingerprints with human isolates at 344bp, indicating the zoonotic hazards of Campylobacter jejuni in Egypt.

[Barakat A. M. A.; Mona M. Sobhy; El Fadaly, H. A. A.; Nagwa S. Rabie; Nashwa O. Khalifa; Eman S. Ramadan; Kotb, M. H. R.; Zeinab M. S. Amin Girh; Dalia M. Sedik and Mona S. Zaki. Zoonotic Hazards of Campylobacteriosis in some areas in Egypt. Life Sci J 2015;12(7):9-14]. (ISSN:1097-8135). http://www.lifesciencesite.com. 2. doi:10.7537/marslsj120715.02

 

Keywords: Campylobacter, milk product, fluorescence, prevalence, PCR

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Use of Multiple Regression Analysis to Identify Factors that Affect the Unemployment Rate in the Kingdom of Saudi Arabia

 

Rami Alamoudi, Mohammed Balubaid, Amir Siddiqui

 

Department of Industrial Engineering, King Abdulaziz University, Jeddah, Saudi Arabia

mbalubaid@kau.edu.sa

 

Abstract: This study investigates factors that affected the unemployment rate in the Kingdom of Saudi Arabia form 1999 to 2013. The research was conducted using multiple regression analysis. The factors selected for the study were Domestic Investment, Foreign Investment, Gross Domestic Product (GDP), Saudi Population Growth, Average Individual Income, Number of Industries, Inflation Rate, Bachelor Graduates, Intermediate Diploma Graduates and High Diploma Graduates. The unemployment rate was used as the dependent variable; remaining variables were used as independent variables. The results of the regression analysis indicated significant impact of three variables: GDP, Saudi Population Growth and Inflation Rate. GDP and Inflation Rate were observed to have a negative impact on the unemployment rate, while Saudi Population Growth had a positive relationship with the unemployment rate.

[Rami Alamoudi, Mohammed Balubaid, Amir Siddiqui. Use of Multiple Regression Analysis to Identify Factors that Affect the Unemployment Rate in the Kingdom of Saudi Arabia. Life Sci J 2015;12(7):15-22]. (ISSN:1097-8135). http://www.lifesciencesite.com. 3. doi:10.7537/marslsj120715.03

 

Keywords: Unemployment, multiple regression analysis, Gross Domestic Product (GDP)

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Sonocatalytic degradation of the insecticide parathion in water

 

Abdolmajid Fadaei1* ,and Hamaid Karyab2

 

1Department of Environmental Health Engineering, School of Health, University of Medical Sciences, Shahrekord , Iran

2Department of Environmental Health Engineering, School of Health, QazvinUniversity of Medical Sciences, Qazvin, Iran

* Corresponding Author: Abdolmajid Fadaei, Department of Environmental Health Engineering, School of Public Health, Shahrekord University of Medical Sciences, Shahrekord, Iran., Tel: +98-381-3330299, Fax: +98-381-3334678, Email: ali2fadae@yahoo.com

Email: ali2fadae@yahoo.com

 

Abstract: This work discusses the effects of ultrasound (US) irradiation on the decomposed of parathion in US/ZnO system. The influences of initial concentration of catalyst, initial parathion concentration, irradiation time, and temperature was studied, Gas chromatography mass spectroscopy (GC – MS) was used for analyses of pesticide. The decomposed ratio of parathion increased with the ZnO dosage in 200-–800 mg/l in the UV/ZnO system. It was found out that the optimal concentration of catalyst was 600 mg/l .Results showed that the degradation of pesticide increased with increasing temperature and decreased with increasing pesticide concentration. At 100 min, the decompose ratio of ZnO, US and US/ZnO were 30.2%, 47.6%, 70.2%, respectively. The experimental results showed that the US/ZnO system cannot only completely decomposed but can effectively degradation parathion.

[Abdolmajid Fadaei, and Hamaid Karyab. Sonocatalytic degradation of the insecticide parathion in water. Life Sci J 2015;12(7):23-28]. (ISSN:1097-8135). http://www.lifesciencesite.com. 4. doi:10.7537/marslsj120715.04

 

Key words: sonocatalytic degradation; parathion; zinc oxide.

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Immunohistochemical Profile of Calponin, 14-3-3 σ and Maspin in Salivary Gland Neoplasms

 

Wael Y. Elias

 

Faculty of Dentistry, King Abdulaziz University, Jeddah 21543, Saudi Arabia

dreliasw@gmail.com

 

Abstract: Salivary gland tumors are a morphologically and clinically diverse group of neoplasms which may present significant diagnostic and management challenges. This study aims to investigate the expression of calponin, 14-3-3σ, and maspin proteins in their role as myoepithelial cell markers within malignant salivary gland neoplasms, derived from myoepithelial cells (MECs). Twenty formalin-fixed, paraffin-embedded specimens of malignant salivary gland neoplasms (11 adenoid cystic carcinoma, 4 epithelial-myoepithelial carcinoma and 5 pleomorphic adenoma), were used in this study. For immunohistochemical staining, a peroxidase-antiperoxidase technique was utilized.  The area fraction of immunopositivity for four different microscopic fields was measured and then used to calculate the mean area fraction. Immunohistochemical and image analysis results of the present study revealed a higher calponin, 14-3-3σ and maspin expression in malignant pleomorphic adenoma, with intermediate expression seen in adenoid cystic carcinoma and the least expression detected in epithelial-myoepithelial carcinoma. It may be assumed that myoepithelial cells play a role in tumor development in malignant salivary gland neoplasms, where MECs exist together with luminal cells.

[Wael Y. Elias. Immunohistochemical Profile of Calponin, 14-3-3 σ and Maspin in Salivary Gland Neoplasms. Life Sci J 2015;12(7):29-34]. (ISSN:1097-8135). http://www.lifesciencesite.com. 5. doi:10.7537/marslsj120715.05

 

Keywords: Calponin; 14-3-3σ; maspin; malignant salivary gland neoplasms

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Value of Transvaginal Cervical Ultrasonographic Assessment and Bacterial Vaginosis in Prediction of Preterm Birth

 

Mohamed M. Fahmy, Sherief M.S. Abd El Salam, Mohamed A. Emarah and Abeer A. ElSobky

 

Department of Obstetrics & Gynecology, Faculty of Medicine, Menoufia University, Menoufia, Egypt

ah_haleeem2002@yahoo.com

 

Abstract: Objective: To determine the diagnostic value of cervical length measurement, in the second trimester of pregnancy and impact of bacterial vaginosis as preterm labour (PTL) predictor. Background: Preterm birth is the leading direct cause of neonatal death and morbidity and imposes large costs to the health care system. Early detection of pregnant women at risk of PTL will help to reduce the occurrence of prematurity- related mortality and morbidity. Cervical insufficiency and bacterial vaginosis are two items that recently known play an essential role in preterm delivery. They can be diagnosed using safe, simple and reliable methods. Patients and Methods: This a prospective cohort study included 580 pregnant women uncomplicated singleton pregnancy between 22 and 24 weeks of gestation to assess cervical length which was measured by transvaginal probe immediately after collecting vaginal swab for diagnosis of bacterial vaginosis by Amsel’s criteria. Cervical length (CL) < 30mm was considered short cervix and suggest cervical insufficiency. The vaginal swab had 3 out of 4 Amsel’s criteria was considered positive for bacterial vaginosis. Patients were followed until delivery. Results: Our final analysis was based on results for 500 participants. The incidence of PTL was 100/500 (20 %). The incidence of PTL had bacterial vaginosis (BV) were 52/100 (52%) which is statistically significant. The incidence of PTL in cases with short CL ≤ 30mm were 39/100 (39%) while short cervix cases in full term were 3/400 (0.8%) which is statistically significant. The incidence of PTL were (16%) for cases had BV and short CL while no cases for full term labour which is statistically significant (P < 0.001). Conclusion: The assessment of cervical length by transvaginal ultrasound and detection of bacterial vaginosis during pregnancy have significant value in prediction of PTL.

[Mohamed M. Fahmy, Sherief M.S. Abd El Salam, Mohamed A. Emarah, Abeer A. ElSobky. Value of Transvaginal Cervical Ultrasonographic Assessment and Bacterial Vaginosis in Prediction of Preterm Birth. Life Sci J 2015;12(7):35-40]. (ISSN:1097-8135). http://www.lifesciencesite.com. 6. doi:10.7537/marslsj120715.06

 

Key Words: Bacterial vaginosis, cervical length measurement, Preterm birth, Second trimester.

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Clinical Pathway versus Traditional Care Plan method for Caring of Postoperative Children Undergoing Cardio thoracic Surgery

 

Hyam R. Tantawi1, Inas Lotfy2, Amira Abdallh2 and Bothyna N. Sadek1

 

1Departments of Pediatric and 2Administration Nursing Ain Shams University and Academic Military

hyam.tantawi@yahoo.com

 

Abstract: A clinical pathway is developed to be the ideal plan of care for post operative children undergoing to cardio thoracic surgery. Similarly, some conditions and procedures may have more variable outcomes than others. Although the heterogeneity of children and procedures are important factors in considering the potential impact of clinical pathway. Aim: This study aimed to compare between the traditional nursing care and clinical pathway in planning for caring of postoperative children undergoing cardio thoracic surgery. Methods: A quasi-experimental design was utilized to conduct this study, the study was carried out at Abou-El Resh Talabah Hospital affiliated to Health Insurance and El-Demerdash Hospital affiliated to Ain Shams University Hospital. Sample: Nurses group: A convenience sample consisted of 100 nurses caring for post operative children undergoing cardiothoracic surgery at the previously mentioned study settings. Patients group: A convenience sample of 200 children who were admitted for cardiothoracic surgery in the previously mentioned study settings was recruited for pre and post clinical pathway intervention. Tools of data collection are: Questionnaire format, Observation checklists, Child patient assessment sheet and Clinical pathway map. Results: the percent score of total observation of the care of children patients in the pre-intervention stage study and control groups showed that the scores were very low in both groups. Nonetheless, the score of performance was higher among nurses in the control group (28.83.5) compared to those in the study group. Conclusion: In the light of the current study it can be concluded that, nurses’ knowledge about heart diseases in pediatric is generally low and their performance of postoperative care for post operative open heart surgery is unsatisfied. While after the implementation of the clinical pathway the significant improvements in nurses’ knowledge and performance was observed; all the areas were adequately performed by almost all nurses for almost all children in the study group. This is reflected on child’s outcomes as measured by the length of hospital stay, which was significantly shortened in comparison to pre-intervention stay as well as control group stay. Meanwhile, the clinical pathway team members namely, nurses and resident physician acquired good knowledge about the clinical pathway and the satisfaction level improved post implementation. Recommendations: Further studies should be carried out on a large number of nurses for evidence of results and generalization.

[Hyam R. Tantawi, Inas Lotfy, Amira Abdallh and Bothyna N. Sadek. Clinical Pathway versus Traditional Care Plan method for Caring of Postoperative Children Undergoing Cardio thoracic Surgery. Life Sci J 2015;12(7):41-62]. (ISSN:1097-8135). http://www.lifesciencesite.com. 7. doi:10.7537/marslsj120715.07

 

Key words: Clinical pathway, traditional care, post operative, children, cardiothoracic surgery

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8

The effect of PMF Camel Urine Nanoparticles on A549 Cells: The Mechanism of Action and Drug Delivery

 

Gehan A-R. Ahmed1,2,3*, Faten A. Khorshid4,5, Alaa Khedr6, Salem M. El-Hamidy5, Numan A. Salah7

 

1Biochemistry Department, Science College, King Abdulaziz University (KAU), Jeddah –KSA

2Medical Biophysics Research Lab. -King Fahd Medical Research Centre, KAU, Jeddah –KSA

3Spectroscopy Department, Physics Division, National Research Centre- Cairo, Egypt

4Tissue Culture Research Unit, King Fahd Medical Research Centre, KAU, Jeddah –KSA

5Biology Department, Science College, KAU, Jeddah –KSA

6Faculty of Pharmacy KAU- Pharm. Dep Assuit University-Egypt

7Center of Nanotechnology, King Abdulaziz University, Jeddah-KSA

jahmed@kau.edu.sa, gehan_raouf@hotmail.com

 

Abstract: PMF is a natural selective anticancer, fraction extracted from camel urine (PM701), resemble in their actions the new drugs now used and manufactured in nanomedicine and nanotechnology. The PMF nanoparticles and its effect on the lung cancer cells A549 were studied by scanning electron microscope (SEM), Transmission electron microscope (TEM), energy dispersive X-ray (EDX) and the vibrational spectroscopy. A549 were treated with PMF for 2, 5, 10, 20 seconds and 1 minute. Fourier transforms Infrared Spectroscopy (FTIR) and TEM were used to study the membrane lipid profile and the morphology of the treated cells. The results revealed that several ions that present in a relatively high concentration in PMF were confirmed such as K, Ca, Cd, Y, Eu, Th and zinc, zinc is present in form of ZnO and Ca, Cd & Y are in form of sulfates as confirmed by the oxide and sulfate bands in the FTIR spectrum of PMF. Arginine, hippuric and benzoic acids as well were also detected. The PMF contains different types of macro and nanoshells with different types of metals that attack first, the A549 cell membrane and influenced the membrane polarity, packing and the hydrocarbon chain length of the phospholipids. These changes were indicated by the changes in the asymmetric stretching CH2 /CH3, symmetric stretching of membrane CH2/CH3, symmetric stretching CH2 /total lipids ratios respectively. Second, these nanoparticles directed after endocytosis to the nucleus and other cell organelles some of these metals are responsible for the selectivity of PMF fraction to cancer cells only. This new smart drug based on the most new frontiers in nanotechnology which include nanoshells as drug carrier.

[Gehan A-R. Ahmed, Faten A. Khorshid, Alaa Khedr, Salem M. El-Hamidy, Numan A. Salah. The effect of PMF Camel Urine Nanoparticles on A549 Cells: The Mechanism of Action and Drug Delivery. Life Sci J 2015;12(7):63-75]. (ISSN:1097-8135). http://www.lifesciencesite.com. 8. doi:10.7537/marslsj120715.08

 

Keywords: A549 Lung cancer cells, Nanomedicine, PMF, Vibrational Spectroscopy, Membrane lipids, Electon Microscope, LCMS /GCMS, LC-MSMS – IonTrap, Cancer treatment

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Image-based Intelligent Surveillance System with the Robust Universal Middleware Bridge Service

 

Chia-Hsu Kuo 1, Huan-Ming Hsu 2

 

1. Department of Software Engineering, National Kaohsiung Normal University, Kaohsiung, Taiwan

2. School of Electronics and Computer Science, University of Southampton, United Kingdom

kuoch@nknu.edu.tw

 

Abstract: Considerable emphasis has recently been placed on surveillance topics and homeland security because of the prevalence of terrorist attacks over the past decade. The development of surveillance equipment is progressing from analog CCTV cameras (which were prevalent in the early days) toward digital IP cameras (which are currently prevalent). Image/object surveillance topics have constituted the main interest for many studies in recent years. This paper proposes an image-based intelligent surveillance system (IISS) integrated with the Universal Middleware Bridge Service (UMBS) for IP cam networking. The UMBS provides mechanisms related to manual system setting, automatic configuration, and management for improving the entire setting and installation procedures. The UMBS comprises four main functional modules, namely live video, Playback Video, Intelligent Scheduler, and System Configuration modules. The structure of the robust UMBS ensures adaptability and flexibility in designing and developing intelligent image recognition surveillance systems based on IP cam networking. Therefore, in this study, an efficient implementation of the IISS integrated with the robust UMBS was achieved. Furthermore, an image/object recognition and matching service was implemented for the IISS with UMBS.

[Chia-Hsu Kuo, Huan-Ming Hsu. Image-based Intelligent Surveillance System with the Robust Universal Middleware Bridge Service. Life Sci J 2015;12(7):76-87]. (ISSN:1097-8135). http://www.lifesciencesite.com. 9. doi:10.7537/marslsj120715.09

 

Keywords: Image Recognition; Middleware; Universal Middleware Bridge Service (UMBS); IP Camera; Platform

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Feasibility Study of Tourism Development Impacts on Stable Regional Development Dimensions: The Case Study

 

Rahim Abdollah Fam1 and Parviz Kesavarz2

 

1 Department of Social Science, Bonab Branch, Islamic Azad University, Bonab, Iran.

2 (Corresponding Author), Department of Management, Bonab Branch, Islamic Azad University, Bonab, Iran.

E-mail: parviz.keshawarz@gmail.com.

 

Abstract: Without a doubt one of the most important challenges the region programmers are facing in many countries are issues related to sustainability and sustainable regional development. There are several factors that could have influenced the sustainable development in the region. One of these factors could be tourism and its consequences. Comprehensive study of the impact of tourism in the region requires a regional study in the context of sustainable development. District of Kandovan Tourism with over 850 years of being in the western foothills of the Sahand Mountains in the East Azerbaijan province has an influential impact in attracting Tourism and regional & national development by diverse and pristine nature and cultural and historical authenticity. The present study reviews the concepts of sustainable tourism, sustainable development implications of tourism on the region. This method is based on analytic methods for data collection and data archival and field methods have been used. Statistical Society is Tourism and tourists as well as residents and officials in the region that Cochran method is being used to determine the number of samples. Achieved sample size is 341 people from a random distribution of the questionnaire. Results gathered represent that despite positive economic impact on some aspects of the social and environmental aspects, results are often negative that help to regional instability.

[Rahim Abdollah Fam and Parviz Kesavarz. Feasibility Study of Tourism Development Impacts on Stable Regional Development Dimensions: The Case Study. Life Sci J 2015;12(7):88-96]. (ISSN:1097-8135). http://www.lifesciencesite.com. 10. doi: 

10.7537/marslsj120715.10

Keywords: Tourism, Sustainable Development, Sustainable Regional Development, Kandovan Tourism Area

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The role of online Learning in Distance learning

 

Hasan Emami and Saber Geraeili

 

Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

E-mail: hasan7564_E@yahoo.com

 

Abstract: In its original form, teachers using distance education traveled to remote sites and taught a class, or corresponded with students through mail, telephone, or fax machine. Individualized study has been a method of reaching the remote student for some time. Detailed course instructions are sent to the learner who performs the assigned tasks and returns the completed work to the teacher for evaluation and reassignment if necessary. rural women take different responsibility and roles such as producers of crops, ranching and keeping poultry, children education, housekeeping, supervising family economy and managing it, collecting firewood, weaving carpet, so illiterate women who haven’t possibility to utilize mass media properly too, wouldn’t able to do their duties and roles and also wouldn’t be affective to develop rural societies. So importance of education is very critical for rural women especially extensional educations. Approximately in most UN reports, women has been considered as greatest deprived group at human societies, while at global level, about two third of all affairs is done by women. But only one third of all recorded affairs relates to women. And also just 1% of proceeds of estates and assets of world belong to women and two third of illiterates of world are women, however they form 50% of workforce at agriculture part and they produce half of foods at all over the world.

[Hasan Emami and Saber Geraeili. The role of online Learning in Distance learning. Life Sci J 2015;12(7):97-103]. (ISSN:1097-8135). http://www.lifesciencesite.com. 11. doi:10.7537/marslsj120715.11

 

Keywords: education, online Learning

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Synthesis Gas Production from Catalytic Gasification of Saw Dust

 

Aly M. Radwan, Mohamed A. Hamad and Amr Moustafa Singedy

 

Chemical Engineering & Pilot Plant Department, National Research Center, Cairo, EGYPT

radwnnrc@hotmail.com; hamadnrc@hotmail.com

 

Abstract: Production of synthesis gas from gasification of saw dust with oxygen as a gasifying agent in bench scale batch fixed bed reactor was studied. A series of experiments were performed to investigate the effects of different operating parameters on the performance of the gasification process. These include, oxygen/fuel equivalence ratio, reaction temperature, reaction residence time, particle size of saw dust, particle size of the catalyst and the type of catalyst. The catalysts selected are, olivine, USY zeolite, granulated slag, dolomite, cement kiln dust, calcium oxide and house red brick. Over the range of the experimental conditions used, the results obtained indicate that the best operating conditions for a gasification process are: temperature 800C, oxygen to fuel ratio 0.25, reaction time 90 minutes and calcined cement kiln dust as catalyst. The results also show that the product gas from gasification of saw dust with cement kiln dust has higher H2 and CO concentrations.

[Aly M. Radwan, Mohamed A. Hamad and Amr Moustafa Singedy. Synthesis Gas Production from Catalytic Gasification of Saw Dust. Life Sci J 2015;12(7):104-118]. (ISSN:1097-8135). http://www.lifesciencesite.com. 12. doi:10.7537/marslsj120715.12

 

Key Words: Gasification, saw dust, synthesis gas, catalysts

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Syndrome effects of NaCl and Epibrassinolide on certain molecular and biochemical activities of salt-sensitive Phaseolus vulgariscv. Brunco L. grown under in vitro condition

 

E.M. Nafie, A. A. Khalfallahand R. M. Mansur

 

Botany department, Faculty of Women for (Arts, Science and Education) Ain Shams University, Cairo, Egypt

oeetezaz@yahoo.com

 

Abstract: The present investigation, aimed to evaluate the application efficacy of 24-Epibrassinolide to evoke NaCl stress tolerance of Phaseolus vulgaris L. cv. Brunco. In vitro plantlets cultured on MS amended with 24-Epibrassinolide and /or different concentrations of NaCl for 3/10 d were analyzed. Sodium chloride (100,150 mmol) reduced photosynthetic pigments, carbohydrates and antioxidant enzymes, however, the 50 mmol alone and along with 24-Epi improved most previous attributes. Salinity stress seriously causes membrane damage evidenced as hydrogen peroxide and malondialdehyde increment, while applying 24-Epi alone and concomitant with salts reduced membrane damages. Proline showed syndrome relation with stress levels with further increment after hormonal application suggesting photosynthetic machinery protection against oxidative stress. High level of sodium and chlorine ions were recorded under salt stress associated with reduction in potassium, calcium ions accumulation, however, 24-Epi successfully alleviated ions toxicity and restore ions balance. All tested plantlets exhibited changes in the endogenous plantlet hormones. SDS-PAGE explore unique protein 77 KDa expressed under 24-Epi alone. Salt stress along with 24-Epi application imply impact on the expression of specific oxidative stress marker genes like peroxidase and polyphenol oxidase and point to priority roles for PPO over POD isozymes under salt stress. Our findings provide integrated evidence that in vitro 24-Epi application is effective in amelioration of NaCl stress.

[E.M. Nafie, A.A. Khalfallahand R.M. Mansur. Syndrome effects of NaCl and Epibrassinolide on certain molecular and biochemical activities of salt-sensitive Phaseolusvulgariscv. Brunco L. grown under in vitrocondition. Life Sci J 2015;12(7):119-136]. (ISSN:1097-8135). http://www.lifesciencesite.com. 13. doi:10.7537/marslsj120715.13

 

Keywords: Brunco plantlets; Brassinosteroids; salt tolerance; proline; hormones; gene expression

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GDP versus DHAP in relapsed and refractory Hodgkin disease

 

Abeer F Amin, Mervat M Omar, and Rehab F Mohamad.

 

Department of Oncology, Assiut University Hospital

 

Abstract: Background: cisplatin & gemcitabine have synergistic action, high RR, manageable hematological and non-hematological toxicity in patients with solid tumors. Our study aims to asses and compare RR & toxicity of GDP versus DHAP in relapsed and refractory Hodgkin disease. Patients and methods: Fifty eight patients with pathologically proven HL were managed with ABVD as a first line of treatment, relapsed and refractory patients in (40%) of patients were randomized into two groups; group A: Received GDP (gemcitabine 1000 mg/m2 intravenously (IV) on days 1 and 8, dexamethasone 40 mg IV on day 1, 40 mg orally on days 2–4, and cisplatin 75 mg/m2 IV on day 1) & group B: received DHAP (dexamethasone 40 mg days 1–4, cytarabine 2 g/m2 every 12 hours 2 on day 2, cisplatin 100 mg/m2/24 hours on day 1). Results: Response to second line treatment was lower in group A (CR in 25% of patients & RR was 67% of patients) than in group B (CR in 27% of patients & RR was 73% of patients) which due to different sample size 12 versus 11 patients thus GDP had an equal response rate to DHAP. Treatment toxicity was lower in group A (Grade 3/4 nutropenia in (8%), vomiting in (17%) & TCP in (17%) of patients respectively) & compared to treatment toxicity in group B (Grade 3/4 neutropenia in (82%) of patients, vomiting in (27%) of patients & TCP in (64%) of patients). Conclusion: GDP is an active outpatient treatment with a limited treatment toxicity in comparison to DHAP for patients relapsed or refractory Hodgkin’s disease who are unfit for autologous stem cell transplantation.

[Abeer F Amin, Mervat M Omar, and Rehab F Mohamad. GDP versus DHAP in relapsed and refractory Hodgkin disease. Life Sci J 2015;12(7):137-143]. (ISSN:1097-8135). http://www.lifesciencesite.com. 14. doi:10.7537/marslsj120715.14

 

Keyword: GDP, DHAP, Hodgkin disease

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Biochemical Role of Some Nanoparticles in the Production of Active Constituents in Stevia Rebaudiana L. Callus

 

Mohamed H. Hendawey1, Reda E. Abo El Fadl2 and Taher A. Salah El-Din3

 

1. Biochemistry Unit, Plant Genetic Resources Department, Desert Research Center, Matarya, Cairo, Egypt

2. Tissue Culture Unit, Plant Genetic Resources Department, Desert Research Center, Matarya, Cairo, Egypt

3. Nanotechnology and Advanced Materials Centeral Lab, Agricultural Research Center, Giza, Egypt

mhhendawey@yahoo.com

 

Abstract: The use of modern techniques in the field of nanotechnology is one of the important recent trends for improving active constituents production in the field of tissue culture, for using on a commercial scale. Therefore, in this study we examined the effect of three types of nanoparticles (FeNPS, CuNPS and SiNPS) at different concentrations on the production of active constituents in Stevia rebaudiana L. callus. In light of the obtained results, treatment with nanoparticles had a positive effect on dry weights at all concentrations. GSH level changes in stevia callus represent a good indicator of response to treatment with nanoparticles. A slight decrease occurred in the GSH content when stevia callus treated with FeNPS and CuNPS at most concentrations. While, it was increased after treatment with all cocentrations of SiNPS (except 8 ppm). With respect to antioxidant enzymes, it noticed a slight effect on enzymes activity when stevia callus treated with FeNPS and CuNPS. While, there was a clear positive effect when using SiNPS, where a new band for SOD appeared at 0.5 ppm as well as a clear increase in bands intensity at the other concentrations. Also, SiNPS had effective influence on CAT activity, especially at high concentrations. FeNPS and CuNPS had inhibitory effect on active constitute production in stevia callus with the exception of 2 and 8 ppm FeNPS and 8 ppm CuNPS, which recorded the highest values of stevioside content. While, the low concentrations of SiNPS affected positively on stevioside content, the maximum value was recorded when SiNPS applied at rate of 2 ppm. The effect of nanoparticles on minerals content in stevia callus depends on the nanoparticles type and the concentrations used, but in general, SiNPS had a negative effect on the accumulation of some minerals such as Si and Cu. The researchers in this study recommended activating the application of nanotechnology in the agricultural field for the production of active constituents in stevia callus, because of their medical and industrial importance. The study confirms the effectiveness of SiNPS (in some low concentrations), FeNPS and CuNPS (in some high concentrations) for the production of active constituents and associated with antioxidants (enzymatic and non enzymatic). Also, this study draws attention for the need to complete the work on these nanoparticles with new concentrations and new particle sizes.

[Mohamed H. Hendawey, Reda E. Abo El Fadl and Taher A. Salah El-Din. Biochemical Role of Some Nanoparticles in the Production of Active Constituents in Stevia Rebaudiana L. Callus. Life Sci J 2015;12(7):144-156]. (ISSN:1097-8135). http://www.lifesciencesite.com. 15. doi:10.7537/marslsj120715.15

 

Key words: Stevia rebaudiana L. callus, Nanoparticles, Active constituents and Antioxidants.

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Effect of Nigella Sativa and Vitamin E on Some Oxidative / Nitrosative Biomarkers in Systemic Lupus Erythematosus Patients

 

Abeer Shahba 1 , Noha E. Esheba 1, Abd-Allah Fooda 2, Samia El-Dardiry 2, Ayman Wagih2, Omnia el-Deeb2.

 

Departments of: 1 Internal Medicine, 2 Medical biochemistry. Tanta University, Egypt.

abeer.ali2@med.tanta.edu.eg

 

Abstract: Introduction: Although the cause of SLE is likely multifactorial, it has been suggested that the increased production of reactive oxygen species or/and reactive nitrogen species may favor the development of SLE. Thymohydroquinone is the most abundant active ingredients of Nigella sativa. The free radical scavenging capability of TQ is as effective as superoxide dismutase. Vitamin E is the most powerful biological antioxidant. Aim of the work: To assess the co-therapeutic efficacy of supplementation with Nigella Sativa and vitamin E. Patients and methods: 50 subjects were included; 25 patients diagnosed with mild to moderate SLE, and 25 healthy individuals served as control. SLE patients were supplemented with antioxidants (Vitamin E and Nigella Sativa) for three months to assess the impact on some markers of oxidative and nitrosative stress. Results: We noticed a significant increase in the levels of SOD and GSH in SLE patients following treatment with Nigella sativa and vitamin E in comparison to pre-treatment levels (P<0.001 for both). While the levels of IL-10, MDA, NO, iNOS decreased significantly following treatment (P<0.001 for all). Conclusion: The improvement of selected oxidative and nitrosative biomarkers and SLEDAI score favors antioxidant therapy in SLE.

[Abeer Shahba, Noha E. Esheba, Abd-Allah Fooda, Samia El-Dardiry, Ayman Wagih, Omnia el-Deeb Effect of Nigella Sativa and Vitamin E on Some Oxidative / Nitrosative Biomarkers in Systemic Lupus Erythematosus Patients. Life Sci J 2015;12(7):157-162]. (ISSN:1097-8135). http://www.lifesciencesite.com. 16. doi:10.7537/marslsj120715.16

 

Keywords: Effect, Nigella Sativa, Vitamin E, Oxidative, Nitrosative Biomarkers, Systemic Lupus Erythematosus Patients

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Characterization of  chloroplast gene encoding ribosomal protein S4 and trnV/rm16s spacer gene in Capparis spinosa L.

 

Mai A. Abdallah1, Faten Z. Mohamed1, Hala. EL- Atroush3 and Magdy M. Mohamed* 2

 

1Biochemistry Department, Faculty of Science, Zagazig University, Egypt

2 Biochemistry Department, Faculty of Science, Ain-Shams University, Egypt

3 Botany Department, Faculty of Science, Ain Shams University, Egypt

Hala_elatroush@hotmail.com

 

Abstract: Capparis spinosa is a medical threatened plant species growing in Egyptian desert. It's one of the most important species in family Capparidaceae, which established pharmaceutical and medicinal properties. A complete coding sequence of 606bp chloroplast ribosomal protein 4s (rps4) gene of C. spinosa was amplified, sequenced, cloned, and expressed. The isolated gene showed a maximum identity with C. flexuosa ribosomal protein S4 (rps4; 98%) gene and 96% with Nasturtium officinale ribosomal protein S4 (rps4) gene. The information of ORF of this gene was found to be a new gene encodes an 202 amino acids protein fragment with a calculated molecular mass of about 19 kDa. C. spinosa trnV/rm16s spacer is noncoding cpDNA region and highly predictive that might offer a phylogenetic information for comparison between different noncoding cpDNA regions due to its highly conservative region among uncoding genes. Sequence analysis of trnV/ rrn16s spacer observed a 100% identity with 12 plant sequences in GenBank of different phylogenic loci. This data might be useful for determine the localization of C. spinosa among Capers species.

[Mai A. Abdallah, Faten Z. Mohamed, Hala. EL- Atroush and Magdy M. Mohamed. Characterization of chloroplast gene encoding ribosomal protein S4 and trnV/rm16s spacer gene in Capparis spinosa L. Life Sci J 2015;12(7):163-173]. (ISSN:1097-8135). http://www.lifesciencesite.com. 17. doi:10.7537/marslsj120715.17

 

Key words: C. spinosa, rps4 gene, C. flexuosa, Nasturtium officinale

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Assessment of the Role of Insulin Resistance in Cases of Recurrent Unexplained First Trimesteric Miscarriage

 

K. A. Wahba

 

Obstetrics and Gynecology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

karimwahbaobgyn@yahoo.com

 

Abstract: Objective: To assess the role of insulin resistance in cases of recurrent unexplained 1st trimesteric miscarriage and to show whether investigations for insulin resistance should be added to the routine investigations of recurrent miscarriage or not. Design: A prospective case control study. Setting: The recurrent miscarriage clinic in a tertiary center (Ain Shams University Maternity hospitals). Population: Cases were 180 non-pregnant women with recurrent primary or secondary unexplained 1st trimestric miscarriage (≥ 3 consecutive 1st trimestric miscarriages). Controls were 180 non-pregnant women that matched the cases regarding their age, BMI and had a completely normal obstetric history. Methods: All cases and controls had full assessment regarding their history and examination. Investigations for fasting blood glucose, fasting insulin and full lipid profile were undertaken for all. Outcomes: Primary outcome included fasting insulin levels. Secondary outcomes included fasting blood glucose, fasting glucose-insulin ratio and HOMA calculator results (including insulin resistance, B-cell function and insulin sensitivity). Abdominal girth at the umbilicus, positive family history of D.M., abnormal lipid profile and previous history of macrosomia were also considered. Results: No significant difference in age, BMI and abdominal girth were found between cases and controls. The mean fasting blood glucose, fasting insulin, HOMA insulin resistance & HOMA B cell function was significantly higher in cases in comparison to controls. The HOMA insulin sensitivity was significantly lower in cases in comparison to controls. 26.11% of cases had fasting glucose – insulin ratio <4.5 in comparison to 11.67% of controls and this was highly significant. Conclusion: Frequency of insulin resistance is significantly high in cases with recurrent unexplained 1st trimestric miscarriage. Insulin resistance may probably be an important cofactor for causing recurrent pregnancy loss. Recommendations: A further study will be done on the cases in this study to assess the effect of Metformin intake during pregnancy to improve the pregnancy outcome.

[K. A. Wahba. Assessment of the Role of Insulin Resistance in Cases of Recurrent Unexplained First Trimesteric Miscarriage. Life Sci J 2015;12(7):174-181]. (ISSN:1097-8135). http://www.lifesciencesite.com. 18. doi:10.7537/marslsj120715.18

 

Keywords: insulin resistance, fasting glucose, fasting insulin, recurrent miscarriage

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The following manuscripts are presented as online first for peer-review, starting from May 28, 2015. 

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